Friday, December 27, 2019
Biology Chemical Tests and Test - 2049 Words
Biology 100 Lab Laboratory Report Title: Identifying the four major classes of macromolecules through various tests. Group Members: Antonia Johnson, Payton Ewing, Lenneisha Hepburn, Kendisha Hanna, Introduction: Macromolecules also known as biological molecules are monomers which are the simple units of polymers. All macromolecules contain carbon and hydrogen which are found in organic compounds. In this Experiment we will test for the presence of protein, starch, lipids, and sugars by analyzing the content off food and / or other substances by utilizing different testing methods. In the scientific method, a chemical test that is sensitive to these groups can be used to identify molecules that are in that class. Testing involvesâ⬠¦show more contentâ⬠¦To the same test tube 10 drops of Sudan IV dye was added. The test tube was then shaken and allowed to set for 1 minute. The test tube was then carefully observed for the distribution of dye, water and salad oil. The results were then recorded and the experiment was repeated with the remaining substances. The Grease Spot test (test for lipids ) An unglazed paper was obtained and several drops of food item/substance to be tested were then added. An observation was then made for a translucent grease mark on the paper. Results were then recorded Results Biuret Test Test tube Number | Biuret reagent | Substance to be tested | Color Before | Color after the substance was added | 1 | 10 drops | 1ml of 10% albumin | Blue | lilac | 2 | 10 drops | 1ml of milk | Blue | lavender | 3 | 10 drops | 1ml of potato | Blue | clear | 4 | 10 drops | 1ml 50% egg albumin | Blue | purple | 5 | 10 drops | 1ml boiled bean mixture | Blue | Rusty brown | 6 | 10 drops | 1ml 10% sucrose | Blue | Clear | 7 | 10 drops | 1ml unknown | Blue | Yellow/green | 8 | 10 drops | 1ml control | Blue | lilac | 9 | 10 drops | 1ml salad oil | Blue | Emissible (did not mix) | 10 | 10 drops | 1ml Chicken (cooked) | Blue | Lavender | Iodine test (test for starch) Test Tube Number | Drops of Iodine | Substance to be tested | Color Before the substance was added | Color After the substance was added | 1 | 4 | Potato | Brown | Black | 2 | 4 |Show MoreRelatedThe Influence of Light on Photosynthesis1500 Words à |à 6 Pagesprocess of converting light energy into chemical potential energy and storing it as starch. 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